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2 pages/≈550 words
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MLA
Subject:
Technology
Type:
Book Report
Language:
English (U.S.)
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MS Word
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Topic:

Paper Chromatography (Book Report Sample)

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Instructions: Hello, I want you to write a lab report for chemistry. I have attached everything related to this lab report with the checklist to follow.The objective of this experiment is to separate mixtures into their different components to identify and analyze the components of the mixture. Carefully analyze the color changes in relation to the various acid components. The Sample: The main hypothesis is to test whether amino acids can be successfully separated into two unknown mixtures through the process of paper chromatography. This experiment’s significance is widely applicable in pharmaceutical industries whereby the process is vital in preparing large volumes of pharmaceutical compounds, testing the level of purity for trace contaminants, ad separating chiral drugs such as thalidomide, sleeping peel and an antidote for morning sickness in pregnant women. The process is also applicable in hospitals to decipher the level of alcohol in the blood of a patient and perform occupation hygiene investigations such as contamination of surface by specified antineoplastic drugs. Thirdly, the procedure is helpful in forensic science to carry out an analysis of body fluids for illegal substances and to compare a sample obtained at a scene of crime to samples from suspects e.g. blood and fibre. In environmental science, the procedure is used to determine the pollution levels in air and drinking water for instance pesticides. Lastly, the process is employed in manufacturing plants to purify chemical needed to process a product such as pesticides.

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Content:
Paper Chromatography
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Abstract
The objective of this experiment is to separate mixtures into their different components to identify and analyze the components of the mixture. The results will depict purple spots corresponding to where the amino acids are located.
Introduction
The main hypothesis is to test whether amino acids can be successfully separated into two unknown mixtures through the process of paper chromatography. This experiment’s significance is widely applicable in pharmaceutical industries whereby the process is vital in preparing large volumes of pharmaceutical compounds, testing the level of purity for trace contaminants, ad separating chiral drugs such as thalidomide, sleeping peel and an antidote for morning sickness in pregnant women. The process is also applicable in hospitals to decipher the level of alcohol in the blood of a patient and perform occupation hygiene investigations such as contamination of surface by specified antineoplastic drugs. Thirdly, the procedure is helpful in forensic science to carry out an analysis of body fluids for illegal substances and to compare a sample obtained at a scene of crime to samples from suspects e.g. blood and fiber. In environmental science, the procedure is used to determine the pollution levels in air and drinking water for instance pesticides. Lastly, the process is employed in manufacturing plants to purify chemical needed to process a product such as pesticides. In this paper chromatography, the procedure involves a mobile phase, which is a liquid, and a stationary phase that is represented by a sheet of paper smeared with a thin layer of absorbed liquid. The paper is applied with the solution of the mixture to be separated as a minute spot towards the lower edge of the paper, and the paper submerged into a shallow layer of the mobile phase. Through capillary attraction, the mobile phase will creep up the paper and flows past the spot.
Materials and Procedures
A toothpick is first placed into the amino acid and spotted on the filter paper. Then the samples spotted on the filter paper are allowed to dry completely. When dried from a cylinder with a filter paper, spots should be located at the bottom of the paper. Then the paper is carefully stapled together, one staple at the top and another at the bottom. The solvent level should be ascertained to be below the line containing mine spotted amino acids. The next step involves allowing the chromatography to develop until the solvent line is 1cm from the top. While the filter paper is still wet, a line is drawn along the top of the solvent using a pencil. After the...
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