Bacterial Endospore (Lab Report Sample)
Write a lab report on Endospore Stain Using Bacillus Subtilis and Capsule Stain Using Enterobacter aerogenes and Staphylococcus aureus. Answer the following questions: What are bacterial endospores, and what is their purpose?
Why is an older culture necessary for staining endospores, and what do positive and negative endospore stain results indicate?
How do bacterial capsules facilitate bacterial adhesion and protect cells from desiccation?
What type of capsules do some bacteria produce, and why is heat fixation not recommended for staining bacteria in capsules?
What happens to the background and cells of a bacterial smear when stained with a solution of eosin and methylene blue?
What is catalase, and how does it protect organisms from oxidative damage?
Student’s Name
Professor’s Name
Course Title
November 24, 2022
Endospore Stain Using Bacillus Subtilis and Capsule Stain Using Enterobacter aerogenes and Staphylococcus aureus
Introduction
In bacterial cells that have experienced nutritional deficiency, endospores are identified and subsequently classified using the Schaefer-Fulton or Endospore stain. Bacillus subtilis was the bacteria utilized in this staining process. The main purpose of capsule stain, also known as negative stain, is to separate bacterial cells from capsular material. Chemicals that are effective with simple stain but not capsule stain cannot be utilized in the process. During this procedure, an acidic dye or stain will be used to check for the presence of a capsule produced by Enterobacter aerogenes and Staphylococcus aureus species.
Materials
Bacillus subtilis (an older culture), Enterobacter aerogenes, Staphylococcus aureus, malachite green, safranin, nigrosine, stain rack, hot plate, slides, microscope.
Methods
Endospore Stain
First of all, a Bacillus species smear was formed, air-dried, and heat fixed. Water was placed in a beaker and
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