Change of Temperature Effects on Yeast Cells Absorption (Lab Report Sample)

Change of temperature effects on yeast cells absorption
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Change of temperature effects on yeast cells absorption
Introduction
The effect of temperature on the absorption of yeast cells has been investigated for several years. Temperature is one of the major factors that affect the growth of yeast and consequently the yeast cells' absorption (Akinbobola, 2019). Just like other chemical reactions, yeast cells' absorption is a chemical reaction that is influenced directly by temperature. The rate of yeast cells’ absorption increases as temperature is increased above the room temperature up to 40 degrees Celsius (Akinbobola, 2019). Yeast depends on sugar to produce carbon dioxide for purposes of fermentation. Notably, yeast ferments sugar, producing a waste product in form of carbon dioxide (Ciani et al., 2016). There is a direct relationship between temperature and yeast cells absorption; as the temperature increases the rate of yeast cells also increases.
Materials and Methods
0.06 g of Saccharomyces cerevisiae was added to 3ml of yeast growth medium. The yeast growth medium consisted of 0.62gm of HEPES which was added into 1.008 gm of glucose, placed on a weight reader. 100 ml of distilled water was then added to the solution. To adjust the pH of the solution, a small amount of HCL was added into the whole solution and everything was mixed.
Further, 9ml of Yeast growth medium was added to 15ml of the centrifuge tube and dilutes by 1ml of 2% yeast suspension, then mixed properly. The working concentration of the resulting suspension was calculated and the yeast suspension was allowed to settle for 12 minutes.
Fungal structures are usually less visible without staining, thus Neutral Red Dye was used for staining. Neutral Red dye (NRD) was prepared in microtubes by adding 200 ul of yeast suspension, and the microtubes were incubated for 30 minutes at room temperature to allow the transport of NRD to take place in the yeast cells. The yeast cell suspensions were then washed to remove excess NRD.
Results
The rate of yeast cells absorption increases as the temperature is increased from 00C and reaches a maximum of 400C (from 0.316 to 0.487). The optimal temperature for the rate of absorption for the yeast studied was found to be 400C. Maximum cell absorption took place at this temperature.
Table 1: Experimental Results given variation in Temperature
 

 

Absorption Rate

 

 

Temperature

 

Exp 1

Exp 2

Exp 3


40

0.442

0.598

0.422


30

0.35

0.389

0.385


22.5

0.354

0.369

0.371


10

0.387

0.324

0.359


0

0.175

0.418

0.354

 

 

 

 

 

In the experiment, the temperature was varied during the incubation time. When the temperature was increased above room temperature 22.50C, the rate of yeast absorption also increased. Also, when the temperature was decreased below room temperature the rate of absorption by yeast cells decreased.
Table 2: Mean and Standard Deviation
Temperature

Mean

Standard Deviation

40

0.487

0.102

30

0.375

0.021

22.5

0.365

0.009

10

0.357

0.032

0

0.316

0.126

Table 3: Graph showing mean of different temperatures