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1 page/≈275 words
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APA
Subject:
Health, Medicine, Nursing
Type:
Lab Report
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English (U.S.)
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Topic:

Biochemical Identification of the Unknown Health, Medicine Lab Report (Lab Report Sample)

Instructions:

LABORATORY REPORT ON Biochemical Identification of the Unknown

source..
Content:


Laboratory Report: Biochemical Identification of the Unknown
Student Name
Institutional Affiliation
Laboratory Report
Biochemical Identification of the Unknown

Unknown

Gram Stain

TSA

PEA

MAC

Purpose


This test is used to separate the main two groups of bacteria based on the constituents on their wall (Mandal, Tabassum, Shuvo & Habib, 2019). Gram-positive walls are composed of a peptidoglycan layer that is thick.

This is a differential test that consists of lactose, sucrose minimum quantity of dextrose (glucose), ferrous sulfate, and pH indicator phenol red.

This test selects Gram-positive organisms facilitating their growth. It's composed of phenyl ethyl alcohol (significantly decreases Gram-negative organisms growth by obstructing DNA synthesis)

Selective and differential medium. It distinguishes the gram-negative enteric bacteria lactose fermenters from the non-lactose fermenters (Mandal, Tabassum, Shuvo & Habib, 2019).

Observation

Organism A

Pink colored short rods bacteria

yellow slant/red butt

No growth identified

Growth of pink colonies with a precipitated zone.


Organism B

Violet colored spherical shaped bacteria

Yellow slant /yellow butt

Growth was seen

No growth

Conclusion

Organism A

Gram-negative bacteria

The organisms are lactose, glucose, and sucrose non-fermenters.

Gram negative

Gram Negative Lactose fermenter.


Organism B

Gram-positive bacteria

The organisms ferment lactose sucrose and glucose.

Gram-positive

Gram-positive bacteria

Based on the above results for the differential and selective medium, they support the gram staining results. According to Mandal, Tabassum, Shuvo & Habib (2019) the PEA medium allows only the thriving of gram-positive bacteria, while MacConkey facilitates gram-negative only to thrive.
Gram-Negative bacteria: Klebsiella pneumoniae
Tests

Purpose

Media &Reagents

Observation and Conclusion

Indole

Based on the principle of identifying bacteria that convert tryptophan to indole

P-Dimethylaminobenzaldehyde
37% of Hydrochloric Acid

...
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