Biochemical Identification of the Unknown Health, Medicine Lab Report (Lab Report Sample)
LABORATORY REPORT ON Biochemical Identification of the Unknown
source..
Laboratory Report: Biochemical Identification of the Unknown
Student Name
Institutional Affiliation
Laboratory Report
Biochemical Identification of the Unknown
Unknown
Gram Stain
TSA
PEA
MAC
Purpose
This test is used to separate the main two groups of bacteria based on the constituents on their wall (Mandal, Tabassum, Shuvo & Habib, 2019). Gram-positive walls are composed of a peptidoglycan layer that is thick.
This is a differential test that consists of lactose, sucrose minimum quantity of dextrose (glucose), ferrous sulfate, and pH indicator phenol red.
This test selects Gram-positive organisms facilitating their growth. It's composed of phenyl ethyl alcohol (significantly decreases Gram-negative organisms growth by obstructing DNA synthesis)
Selective and differential medium. It distinguishes the gram-negative enteric bacteria lactose fermenters from the non-lactose fermenters (Mandal, Tabassum, Shuvo & Habib, 2019).
Observation
Organism A
Pink colored short rods bacteria
yellow slant/red butt
No growth identified
Growth of pink colonies with a precipitated zone.
Organism B
Violet colored spherical shaped bacteria
Yellow slant /yellow butt
Growth was seen
No growth
Conclusion
Organism A
Gram-negative bacteria
The organisms are lactose, glucose, and sucrose non-fermenters.
Gram negative
Gram Negative Lactose fermenter.
Organism B
Gram-positive bacteria
The organisms ferment lactose sucrose and glucose.
Gram-positive
Gram-positive bacteria
Based on the above results for the differential and selective medium, they support the gram staining results. According to Mandal, Tabassum, Shuvo & Habib (2019) the PEA medium allows only the thriving of gram-positive bacteria, while MacConkey facilitates gram-negative only to thrive.
Gram-Negative bacteria: Klebsiella pneumoniae
Tests
Purpose
Media &Reagents
Observation and Conclusion
Indole
Based on the principle of identifying bacteria that convert tryptophan to indole
P-Dimethylaminobenzaldehyde
37% of Hydrochloric Acid
Other Topics:
- Individual Responsiveness Against Antigen, What is Antibodies, and ImmunoglobulinsDescription: Specificity and diversity immune responses are specific for distinct antigens. Memory exposure of the immune system to a foreign antigen enhances its ability to respond again to that antigen. Rsponses to second and subsequent exposures to the same antigen called secondary immune responses are usually more ...1 page/≈550 words| 3 Sources | APA | Health, Medicine, Nursing | Lab Report |
- Factors that determine Responsiveness of an Individual against AntigenDescription: Memory exposure of the immune system to a foreign antigen enhances its ability to respond again to that antigen. Rsponses to second and subsequent exposures to the same antigen called secondary immune responses are usually more rapid larger and often qualitatively different from the first exposure....1 page/≈550 words| 8 Sources | APA | Health, Medicine, Nursing | Lab Report |
- Comprehensive Health History and Physical AssessmentDescription: The patient presented himself, and he seems reliable. Additionally, the assessment relies on an old California Pacific Medical Center chart. The patient has a good memory and trusts me completely. Therefore, the information collected has a perfect score of 89%....8 pages/≈2200 words| 4 Sources | APA | Health, Medicine, Nursing | Lab Report |
