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Pages:
3 pages/≈825 words
Sources:
1 Source
Level:
MLA
Subject:
Life Sciences
Type:
Lab Report
Language:
English (U.S.)
Document:
MS Word
Date:
Total cost:
$ 10.8
Topic:

Caenorhabditis Elegans Gene Expression Report Assignment (Lab Report Sample)

Instructions:

A LAB REPORT OF C. ELEGANS LACZ GENE EXPRESSION

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Content:
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Caenorhabditis elegans Gene Expression Lab Report
Introduction
C. elegans is a multicellular organism with a relatively shorter life span, 3 days. This allows researchers to employ it in several kinds of studies because it will take a short period of time for results to be observed. In addition they are inexpensive in terms of maintenance requirements. Their tiny size, of approximately 1mm, demands for little space in a limited laboratory space. It is relatively transparent thus it allows for the observation of internal body structures as well as staining patterns (Boulin, Thomas, Etchberger, and Hobert, n.pag.). `
C. elegans can be visualized for differential expression patterns of reporter genes by application of the green fluorescent proteins and the LacZ gene for the expression of beta-galactosidase. This experiment concentrates on differential expression of LacZ gene. The worm’s transparent nature will allow for microscopic examination of the worm without the need for dissection (Boulin, et al. n. pag.).
The disadvantage of using the LacZ gene as a reporter gene in C. elegans is that the worms have to be killed and fixed first before staining and visualization processes. In the other hand the green fluorescent protein allows for visualization on life organisms. In that respect the LacZ gene is not a good candidate for gene expression pattern examination; localization of target proteins and cellular or multicellular anatomical studies (Boulin, et al. n.pag.).
Materials and Methods
For this paper, I did adhere and followed the guidelines for materials and procedures as it is in the laboratory manual without any alteration whatsoever.
Results and Discussion
The aim of this paper was to examine for the differential expression patterns of the LacZ reporter gene in C. elegans. I was also required to identify mutant strains basing on the differential patterns of expression of the LacZ reporter gene in C. elegans. I used the X-gal substrate which turned blue based on the differential quantities of the β-galactosidase, a product of the LacZ gene expression. The X-gal substrate turned blue when it interacted with the enzyme named above. However, I noted some differences on the co...
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