Evaluating The Antibacterial Activity in Eugenia Caryophyllata Leaf and Stem Extracts (Research Paper Sample)

Project Title: Evaluating The Antibacterial Activity in Eugenia caryophyllata Leaf and
Stem Extracts
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Clove plant (Eugenia caryophyllata L.) has extensive applications in the food industry. Recent studies have shown that the plant possesses antiseptic properties. Besides, several herbalists have claimed to use the leaves and the stem-bark in the treatment of wound infections. Clove plant root extracts have revealed anti-microbial properties (Kuang et al. 294). However, there is no scientific report showing the antibacterial properties of stem and leaves extracts. This lack of scientific knowledge has created an interest towards carrying out a research that will fully reveal the antibacterial property of the plant. This work will show therapeutic potentials of clove as a cheap antibiotic in the field of medical.
Cloves root extracts are efficient at creating an environment that is hostile to harmful organisms (Barakat and Hassan 1934). The plant extract has been found to hinder Giardia growth showing a potential natural therapy against giardiasis. Clove, cinnamon, as well as peppermint, are also some of the vital oils that have expressed action against pathogenic microbes. Likewise, Japanese researchers have unveiled an interesting use of clove plant. It is apparent that clove can offer seafood with protection against disease-causing Microorganisms (Lee et al. 16). Clove oil can be used as an insect repellant for people who want to elude conventional toxic contacts. Studies have also revealed that clove oil can be an effective mosquito repellant.
Materials and Methods
MaterialSource of materialQuantityUnit Cost($)Total cost($)Leaves and stems of Eugenia caryophyllataState Botanical garden100 grams$ 5$10Whatman filter paperLife technologies1$ 10$ 10Membrane filterLife technologies1$ 20$ 20Culture Media and growth hormonesBiotechnologies
Huntsville1 $ 20$ 20Laboratory ReagentsLife technologies500 grams$ 20$ 20Disposable Petri DishesLife technologies20$ 1$ 20 Total cost of proposed research $ 100
Experiment 1: Preparation of extracts
Fresh leaves and stems of Eugenia caryophyllata will be obtained from the Botanical garden. The extracts will be air-dried at room temperature in a dark room. A few extracts will then be ground into powder. Approximately 30 g of powdered sample will be boiled in 200 ml of water for 60 min. And then filtered through Whatman filter paper and then through a membrane filter. The filtrate will then be evaporated to dryness at room temperature in a constant air current and the product recorded as a percentage of the quantity in 30 g used. The dried extract will then be sterilized by for 12 hours before reconstituting it onto sterile nutrient agar.
Experiment 2: Test bacterial strains
Strains of Escherichia coli will be obtained from Bio-resource Development and Conservation Centre. Local clinical isolates will be obtained from the Clinical Laboratory (Department of Microbiology). All test strains will be re-isolated three successive times on nutrient agar to ensure pure isolates are used.
Experiment 3: Determination of the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC)
The purified E. coli samples from Bio-resource center will then be inoculated onto nutrient agar containing clove extract (T1) with a control (T2). Equally, samples from Clinical Laboratory will then be inoculated onto nutrient agar containin...