About The Procedures,calculate Volume Of Gas Production (Lab Report Sample)

Course:

Biology


Unit:

Plants: Green Factories


Assignment:

Experiment: Photosynthesis


Lab Report Expectations and Instructions:
Lab report grades make up 40% of each unit grade. Before you complete your first lab assignment, please take some time to review the following documents and notes.
Please place your work for each section of the lab report below the instructions for each section on the template. Be sure to leave all directions and questions intact within the template.
Here are your goals for this lesson:
* Make quantitative observations about the effect of light on photosynthesis
* Make quantitative observations about the effect of temperature on photosynthesis
* Make quantitative comparison between the rate of gas exchange between light and dark environments.
** Please read over the teacher comments after the report is submitted and graded.

Experiment: Photosynthesis
Student Name:
Date Experiment done:
Date Report Completed:
Name of Grader:
Hypothesis (10 points):
* A prediction of what you think the results of the project will be before you complete the project.
* Create an if, then statement for each part of the lab that is related to the goal(s) of the experiment. (Example: Goal: To determine what improves student grades. Hypothesis: If students get adequate rest, then grades will improve). 
Hypothesis:

If I provide more light, then the rate of photosynthesis will increase and so will the amount of released oxygen. If the temperature is raised, then the rate of photosynthesis will increase and if the temperature drops, then the rate will decrease.

Procedure (5 points):
* In 1st person past tense, write step by step what you actually did in performing the experiment. 
* Summarize in your own Words. Be sure to include any modifications. 
* You should include enough detail so that someone could reproduce the experiment based on what you have written.
Procedure:

For the first experiment, I filled a flask with 100ml of NaHCo3. Later I put a branch of an Elodea plant in the flask. I attached a thermometer to the flask. I dragged the plant lamp all the way down and put it to 10(maximum light) while I turned the overhead lights in the lab off. After sealing the flask with a rubber-stopper, I attached a syringe to it and set a timer in order to measure the volume of gas produced. I measured the volume of gas at 0,0 minutes first and then at 2.5,5,7.5 and 10 minutes. For the second experiment, the same procedure was followed. However all the lights were off. For the third experiment, the procedure included the same as above with the plant light dimmer set on 8 and then I repeated it while it was set on 4. The temperature for all three experiments was 21,5o C and the plant lamp was as close to the work bench as possible. For the fourth experiment, I made a few changes. I took a flask again and filled it with 100 ml of NaHCo3 and a branch of Elodea plant. I also attached a thermometer. Later I took a constant thermometer bath and placed it on the workbench and put the flask in it while I set the temperature at 40o C. When the thermometer reached a steady temperature of 40o C, I positioned the plant light at the height of the shelf line and set it at 10 (maximum light). The overhead lights were still off. I closed the flask with a stopper and attached a syringe. I set a timer and recorded the gas volume at 0.0,2.5,5,7.5 and 10 minutes. Then I repeated the procedure with the temperature set at 0o C.

Data (30 points):
* Fill out the data chart(s) below by answering the questions or recording data.
* List all measurements and include proper labels and units.
Lab Data: Temperature: 21,5o CPlant type:Elodea plant
Experiment 1: Light Setting:10 (maximum)only the plant lamp was on ,the overhead lights were off
Time (min):Volume (mL)Change in volume (mL)
0.00____
2.524.924.9
5.0 47 22.1
7.5 68.2 21.2
10.0 88.6 20.4
Experiment 2: Light Setting:0, the overhead lights were off
Time (min):Volume (mL)Change in volume (mL)
0.0 88,6______
2.5 85 3,6
5.0 82.7 2.3
7.5 80.4 2.3
10.0 78.2 2.2
Experiment 3:
Light Setting: 8Light Setting 4
Time (min)Volume (mL)Time (min)Volume (mL)
0.01,40.0 1,1
2.522,52.5 16,7
5.043,35.0 32
7.5637.5 47
10.08310.0 61,5
Experiment 4: Light setting: 10
Temp: 40 CTemp: 0 C
Time (min)Volume (mL)Time (min)Volume (mL)
0.00.4 0.0 0
2.592.5 1.7
5.017.45.0 3.2
7.525.87.5 4.7
10.034.210.0 6.3
Data Analysis- Calculations (10 points):
* Show all math performed.
* Type the full calculations in the calculations box
* Give the results in the answer box
* Include proper labels and units
Experiment 1:
1 Find the average of your changes in volume. Average change= (24.9+22.1+21.2+20.4)/4=22.15 ml
2 Divide this value by 2.5 to obtain the rate of O2 production in mL/ minute. 22.15:2.5=8.86 ml/min
Experiment 2:
1 Find the average of your changes in volume. Average change=(3.6+2.3+2.3+2.2)/4=2.6 ml
2 Divide this value by 2.5 to obtain the rate of O2 production in mL/minute. 2.6:2.5=1.04ml/min
Data Analysis- Interpretation Questions (10 points):
* Answer all questions listed below.
Experiment 2:
1 How does this value compare to the value you found in experiment 1? The value is equal to 11.73% of the value I found in experiment 1.
2 What happens to the rate of gas exchange between a light setting of 10 and a setting of 0?The rate of gas exchange decreases dramatically.
Experiment 3:
1 How does your rate of gas production compare between a light setting of 8 and a light setting of 4? The rate of gas production is considerably greater when the light is set at 8.
2 Why is there a difference? Because the more light we provide, the greater the rate of photosynthesis becomes.
Experiment 4:
1 How does the rate of gas production compare between a warm and cold temperature? The rate of oxygen production per minute is 6.944 and 1.272 for a warm and cold temperature, respectively. That means that the gas rate in cold temperature is approximately 18% of that in warm temperature.
2 What does this say about the amount of photosynthesis that is occurring at these two temperatures? That means the amount of photosynthesis in the cold temperature drops significantly and it is almost 1/6 of the amount of photosynthesis in warm temperature.
Conclusion (30 points):
* Answer the following questions in full sentences.
* Helpful tip: someone should be able to read ONLY your conclusion and tell what you thought was going to happen, what actually happened, and why it happened.  It should show complete understanding of the concepts of this lab. Use the three questions below to form your conclusion.
1. What was your hypothesis and was it correct? (2-3 sentences)
I assumed that when the light intensity increases, the rate of photosynthesis increases, as well. My hypothesis was correct as it is proved through experiments 1-3. The rate of oxygen production was maximum when the plant lamp was the closest to the plant and it was set at maximum light. I also assumed that when I raise the temperature, then the rate of photosynthesis will increase and if I decrease the temperature, then the rate will also decrease. My hypothesis was correct as it is proved in experiment 4. The rate of photosynthesis in the cold temperature drops significantly and it is almost 1/6 of the amount of photosynthesis in warm temperature.

1 How do you know your hypothesis was correct? Use relevant data/observations to support your answer. (3-5 sentences)
My hypothesis is proved to be correct if all the first three experiments are taken into consideration. The rate of oxygen production was higher in the first experiment where maximum light was provided and lower when no light was provided in the second experiment. As far as the third experiment is concerned, there is a considerable difference when the light was set at 8 and 4. In the first case there is a higher rate of oxygen production and thus a higher rate of photosynthesis. Also, concerning my second hypothesis, I calculated that the rate of photosynthesis in cold temperature is almost 1/6 of the rate in warm temperature.

2 Go back and read the goals for this lesson at the top of this template. Form a summary statement for each goal, showing you understand and have met the goals of this lab.  Be sure to explain all major concepts and relationships presented in this lab. (3-5 sentences)
To summarize, the rate of photosynthesis increases as the light intensity increases. If we double the light intensity (as in the experiment 3), the rate of gas production per minute increases by approximately 75%. In addition, in a dark environment the rate is 11,73% of the rate when t...